FISH Study: Neoplastic Interphase D9Z1 (Trisomy 9)
- Bone Marrow Aspirate: Obtain 1 - 2 cc of in a greentop sodium heparin tube and immediately rotate it thoroughly to ensure adequate mixing with the anticoagulant.
- Bone Marrow Core Biopsy: Deposit samples in a sterile container containing tissue culture medium with antibiotics (RPMI) that have been brought to room temperature before collection.*
- Peripheral Blood Specimens: Peripheral blood specimens may be submitted in certain clinical conditions (e.g. circulating tumor cells).
- Unacceptable Conditions:
- Frozen specimens
- Specimens for FISH study only > one week old
- Specimens for concurrent FISH and cytogenetic studies > 48 hours old*
- Formalin, alcohol or paraffin-preserved specimens
- Stability (collection to initiation of testing):
- Ambient: 24 - 4By report; interpretive8 hours*
- Frozen: Unacceptable
* Cell viability of specimens requiring cell culture may be compromised in specimens older than 48 hours upon receipt. Refrigeration at 2° - 8° C may assist when a delay is not preventable. Do not freeze or place specimens directly on ice.
This test is used to detect trisomy for chromosome 9, which is a relatively common finding in plasma cell disorders.
Interphase nuclei fluorescence in-situ hybridization (FISH) copy number analyses; a concurrent or previous conventional cytogenetic workup is required/ recommended (where possible)
5 - 12 days
Monday - Saturday