FISH Study: Neoplastic Interphase D9Z1 (Trisomy 9)

Additional Information:


CPT Code(s):

  • 88271
  • 88275
  • 88291

Collection Procedure:


Specimen Requirements:

  • Bone Marrow Aspirate: Obtain 1 - 2 cc of in a greentop sodium heparin tube and immediately rotate it thoroughly to ensure adequate mixing with the anticoagulant.
  • Bone Marrow Core Biopsy: Deposit samples in a sterile container containing tissue culture medium with antibiotics (RPMI) that have been brought to room temperature before collection.*
  • Peripheral Blood Specimens: Peripheral blood specimens may be submitted in certain clinical conditions (e.g. circulating tumor cells).
  • Unacceptable Conditions:
    • Frozen specimens
    • Specimens for FISH study only > one week old
    • Specimens for concurrent FISH and cytogenetic studies > 48 hours old*
    • Formalin, alcohol or paraffin-preserved specimens
  • Stability (collection to initiation of testing):
    • Ambient: 24 - 4By report; interpretive8 hours*
    • Frozen: Unacceptable

* Cell viability of specimens requiring cell culture may be compromised in specimens older than 48 hours upon receipt. Refrigeration at 2° - 8° C may assist when a delay is not preventable. Do not freeze or place specimens directly on ice.


This test is used to detect trisomy for chromosome 9, which is a relatively common finding in plasma cell disorders.


Interphase nuclei fluorescence in-situ hybridization (FISH) copy number analyses; a concurrent or previous conventional cytogenetic workup is required/ recommended (where possible)


5 - 12 days

Reference Values:



Monday - Saturday

Interpretation Data: