FISH Study: Neoplastic Interphase IGH/CCND1
UFHPL Test #: 30200
- Bone Marrow Aspirate: Obtain 1 - 2 cc of in a green-top sodium heparin tube and immediately rotate it thoroughly to ensure adequate mixing with the anticoagulant.
- Bone Marrow Core Biopsy: Deposit samples in a sterile container containing tissue culture medium with antibiotics (RPMI) that have been brought to room temperature before collection.*
- Peripheral Blood Specimens: Peripheral blood specimens may be submitted in those clinical conditions (e.g. circulating blasts).
- Unacceptable Conditions:
- Frozen specimens
- Specimens for FISH study only greater than one week old
- Specimens for concurrent FISH and cytogenetic studies greater than 48 hours old*
- Formalin, alcohol or paraffin-preserved specimens
- Stability (collection to initiation of testing):
- Ambient: 24 - 48 hours*
- Frozen: Unacceptable
* Cell viability of specimens requiring cell culture may be compromised in specimens more than 48 hours old upon receipt. Refrigeration at 2° - 8° C may assist when a delay is not preventable. Do not freeze or place specimens directly on ice.
Plasma cell myeloma causes 20% of deaths from hematologic cancers. This probe is for recurrent genetic abnormalities that can be detected by the most frequent chromosomal fusion translocations involving the immunoglobulin heavy locus.
Interphase nuclei fluorescence in-situ hybridization (FISH) fusion rearrangement analyses; a concurrent or previous conventional cytogenetic workup is required/recommended (where possible).
5 - 12 days
By report; interpretive
Monday - Saturday