FISH Study: Neoplastic Interphase IGH/MAF

Additional Information:

UFHPL Test #: 30210

CPT Code(s):

  • 88271
  • 88275
  • 88291

Collection Procedure:


Specimen Requirements:

  • Bone Marrow Aspirate: Obtain 1 - 2 cc of in a green-top sodium heparin tube and immediately rotate it thoroughly to ensure adequate mixing with the anticoagulant.
  • Bone Marrow Core Biopsy: Deposit samples in a sterile container containing tissue culture medium with antibiotics (RPMI) that have been brought to room temperature before collection.*
  • Peripheral Blood Specimens: Peripheral blood specimens may be submitted in those clinical conditions (e.g. circulating blasts).
Unacceptable Conditions:
  • Frozen specimens
  • Specimens for FISH study only greater than one week old
  • Specimens for concurrent FISH and cytogenetic studies greater than 48 hours old*
  • Formalin, alcohol or paraffin-preserved specimens
Stability (collection to initiation of testing):
  • Ambient: 24 - 48 hours*
  • Frozen: Unacceptable

* Cell viability of specimens requiring cell culture may be compromised in specimens more than 48 hours old upon receipt. Refrigeration at 2° - 8° C may assist when a delay is not preventable. Do not freeze or place specimens directly on ice.


This test is used to detect IGH/GFR3 fusion gene rearrangements, which are most commonly associated with plasma-cell disorders.


Interphase nuclei fluorescence in-situ hybridization (FISH) fusion rearrangement analyses; a concurrent or previous conventional cytogenetic workup is required/recommended (where possible).


5 - 12 days

Reference Values:

By report; interpretive


Monday - Saturday

Interpretation Data: