FISH Study: Neoplastic Interphase MYC
UFHPL Test #: 30230
- Bone Marrow Aspirate: Obtain 1 - 2 cc of in a green-top sodium heparin tube and immediately rotate it thoroughly to ensure adequate mixing with the anticoagulant.
- Bone Marrow Core Biopsy: Deposit samples in a sterile container containing tissue culture medium with antibiotics (RPMI) that have been brought to room temperature before collection.*
- Peripheral Blood Specimens: Peripheral blood specimens may be submitted in those clinical conditions (e.g. circulating blasts).
- Unacceptable Conditions:
- Frozen specimens
- Specimens for FISH study only greater than one week old
- Specimens for concurrent FISH and cytogenetic studies greater than 48 hours old*
- Formalin, alcohol or paraffin-preserved specimens
- Stability (collection to initiation of testing):
- Ambient: 24 - 48 hours*
- Frozen: Unacceptable
* Cell viability of specimens requiring cell culture may be compromised in specimens more than 48 hours old upon receipt. Refrigeration at 2° - 8° C may assist when a delay is not preventable. Do not freeze or place specimens directly on ice.
This test is used to detect MYC gene rearrangements, which are most commonly associated with Burkitt lymphoma but have also been described in both B-cell ALL and in other non-Hodgkin lymphomas.
Note: This test does not identify specific fusion partners but will detect the MYC rearrangements, which may be a variant of the common 8/14 translocation, such as the 8/22 and 2/8 translocations.
Interphase nuclei fluorescence in-situ hybridization (FISH) break-apart rearrangement analyses; a concurrent or previous conventional cytogenetic workup is required/recommended (where possible).
5 - 12 days
By report; interpretive
Monday - Saturday