FISH Study: X/Y Interphase (Bone Marrow)
UFHPL Test #: 30280
- Bone Marrow Aspirate: Obtain 1 - 2 cc of in a green-top sodium heparin tube and immediately rotate it thoroughly to ensure adequate mixing with the anticoagulant.
- Bone Marrow Core Biopsy: Deposit samples in a sterile container containing tissue culture medium with antibiotics (RPMI) that have been brought to room temperature before collection.*
- Peripheral Blood Specimens: Peripheral blood specimens may be submitted in certain clinical conditions if warranted.
- Frozen specimens
- Specimens for FISH study only > one week old
- Specimens for concurrent FISH and cytogenetic studies > 48 hours old*
- Formalin, alcohol or paraffin-preserved specimens
- Ambient: 24 - 48 hours*
- Frozen: Unacceptable
Do not freeze or fix samples in any manner contrary to the maintenance of cell viability. Maintain sample sterility; keep all specimens at room temperature; and deliver samples to the laboratory within 24 - 48 hours. Ship specimens at room temperature for overnight delivery.
* Cell viability of specimens requiring cell culture may be compromised in specimens older than 48 hours upon receipt. Refrigeration at 2° - 8° C may assist when a delay is not preventable. Do not freeze or place specimens directly on ice.
This test is used to monitor sex-mismatched bone marrow and/or stem-cell transplantation status.
Interphase nuclei fluorescence in-situ hybridization (FISH) copy number analyses; a concurrent or previous conventional cytogenetic workup is required/recommended (where possible)
5 - 12 days
By report; interpretive
Monday - Saturday