Container/Tube: Serum gel or EDTA plasma
Sample Volume: Submit full tubes.
Minimum Volume: 0.5 mL serum or plasma
- Send specimens in their original tubes.
- Centrifuge tubes if there is a delay in transport of more than 1 hour.
Storage: Samples are stable for up to 30 days at 2 – 8ºC.
Unacceptable Conditions: Samples with moderate or marked hemolysis will be rejected.
This gamma-glutamyltransferase (GGT) procedure is a modification of the Szasz procedure. GGT catalyzes the transfer of the gamma-glutamyl group from the substrate, gamma-glutamyl-3-carboxy-4-nitroanilide, to glycylglycine, yielding 5-amino-2-nitrobenzoate. The change in absorbance at 410/480 nm is due to the formation of 5-amino-2-nitrobenzoate and is directly proportional to the GGT activity in the sample.
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