Hemoglobin A1C

Additional Information:

UFHPL Epic order code: LAB90

CPT Code(s):


Collection Procedure:

Whole blood, lavender top EDTA tube

Specimen Requirements:

Type: Peripheral blood

Container/Tube: EDTA whole blood

Sample Volume: 0.5mL

Stability (collection to time of analysis/testing):

  • Ambient: 8 hours
  • Refrigerated: 7 days
  • Frozen: 3 months
Specimen Retention Time: 5 days


In a pretreatment step, whole blood is mixed with the Hemolyzing reagent in a 1:100 dilution and the resultant hemolyate is used. The concentration of both HbA1c and total hemoglobin are determined. The HbA1c/Total Hemoglobin ratio is expressed as %HbA1c. Total hemoglobin reagent is used to measure total hemoglobin concentration by a colorimetric method. Change in absorbance is measure at 570/660 nm. HbA1c reagent is used to measure hemoglobin A1c concentration by a turbidimetric immunoinhibition method. In the reaction, hemoglobin A1c antibodies combine with HbA1c from the sample to form soluble antigen-antibody complexes. Polyhaptens from the reagent then bind with the excess antibodies and the resulting agglutinated complex is measured turbidimetrically. Change in absorbance is measured at 340/700 nm.


Within 24 hours

Reference Values:

4.0 – 6.0%