Hepatitis B Surface Antigen
UFHPL Epic order code: LAB471
Hepatitis B virus (HBV) is a DNA virus with a protein coat, the surface antigen (HBsAg) and a nucleic acid core, the core antigen (HBcAg). There are eight different serotypes. Early in infection, HBsAg, HBV DNA, and DNA polymerase can all be detected in serum.
HBsAg can be detected one to seven weeks before liver enzyme elevation or the appearance of clinical symptoms. Three weeks after the onset of acute hepatitis, about 50 percent of patients will still be positive for HBsAg, while at 17 weeks only 10 percent are positive. The best available markers for infectivity are HBsAg and HBeAg. The presence of anti-HBs is frequently associated with noninfectivity. The chronic carrier state is indicated by the persistence of HBsAg and/or HBeAg over long periods (six months to years) without seroconversion to the corresponding antibodies. Such a condition has the potential to lead to serious liver damage, but may be an isolated asymptomatic serologic phenomenon.
Persistence of HBsAg, without anti-HBs, with combinations of positivity of anti-HBc, HBeAg, or anti-HBe indicates infectivity and need for investigation for chronic persistent or chronic aggressive hepatitis.
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Type: Serum or plasma
- If a tube other than a gel-barrier tube is used, transfer the separated serum or plasma to a plastic transport tube.
Sample Volume: 3.5 mL
Minimum Volume: 1.5 mL (Repeat testing is not possible with this specimen volume.)
Storage: Refrigerate specimens immediately after collection.
- Ambient: 14 days
- Refrigerated: 14 days
- Frozen: 14 days
- Freeze/Thaw Cycles: Stable (x 3)
- Non-EDTA plasma specimen
- PST gel-barrier tube