Human Immunodeficiency Virus (HIV) RNA, Real-Time Polymerase Chain Reaction (PCR) (Non-Graph)
UFHPL Test #: 10105
- Acceptable specimens include whole blood or plasma. Blood should be collected in sterile lavender-top tubes, using EDTA as the anticoagulant.
- The minimum required sample volume is 1 mL of plasma or whole blood volume that will produce 1 mL of plasma after centrifugation.
- Samples must be collected aseptically to minimize hemolysis and bacterial contamination.
- Whole blood must be centrifuged within 6 hours of collection at 800 - 1600 x g for 20 minutes at room temperature and can be stored at 2 - 25 °C for up to 24 hours.
- Plasma may be stored at 25 - 30 °C for up to 24 hours and then at 2 to 8°C for up to 6 days or -20 to -80 °C for up to 6 weeks.
- Transportation of whole blood or plasma must comply with country, federal, state and local regulations for the transport of etiologic agents.
- Clotted or frozen whole blood
- Plasma volume less than 1 mL
- Whole blood that is more than one day old that has not been spun
Quantitative HIV-1 RNA level monitoring remains the most important surrogate marker for antiretroviral response.
5 - 8 business days
HIV-1 RNA not detected