Janus Tyrosine Kinase 2 (JAK2 V617F c. 1849 G>T) Gene Qualitative Mutation Analysis
- 83896 x 2
- 83898 x 2
Blood or bone marrow in (lavender top) EDTA or (yellow-top) ACD tube; one 3 mL of blood or 1 mL bone marrow shipped at 4° C is accepted. Severely hemolyzed whole blood or clotted/frozen blood/bone marrow specimen is not accepted.
Cell pellets with at least 10^6-cells, shipped at 4° C, are acceptable
This test is typically ordered to diagnose myeloproliferative neoplasms, including polycythemia vera, essential thrombocythemia or primary myelofibrosis.
Negative (interpretative report provided)
Monday - Friday
- Positive: JAK 2 V617F mutation is detected.
- Negative: JAK 2 V617F mutation is not detected.
A JAK 2 positive indicates the JAK2 mutation at codon 617 is present in the patient’s blood/bone marrow sample; however, a negative result does not necessarily exclude the presence of other JAK2 mutations, such as exon 12/13, or the mutation is less than 2% in the sample. Exon 12/13 mutation analysis is suggested when the JAK 2 V617F is negative.
- Unacceptable Conditions:
- Ambient: Specimens greater than 24 hours old
- Frozen specimens
- Stability (collection to initiation of testing):
- Ambient: 24 hours
- Refrigerated: 72 hours