Lactate Dehydrogenase (LDH)

Additional Information:

UFHPL Epic order code: LAB96

CPT Code(s):


Collection Procedure:

Serum gel tubes should be centrifuged within 2 hours of collection.

Specimen Requirements:

Type: Peripheral blood


  • Serum, gel
  • Plasma, gel

Sample Volume: 0.5mL

Stability (collection to time of analysis/testing):

  • Ambient: ASAP
  • Refrigerated: Not recommended
  • Frozen: Not recommended
Unacceptable Conditions: Moderate or gross hemolysis


The LD procedure employs a modification of the method of Wacker et al. which utilizes the forward reaction of lactate to pyruvate. Lactate and NAD are converted to pyruvate and NADH catalyzed by LD. NADH strongly absorbs light at 340 nm, whereas NAD does not. The rate of change of absorbance at 340 nm is directly proportional to the LD activity in the sample.


Within 24 hours

Reference Values:

140 – 271 U/L