Additional Information:

UFHPL Epic order code: LAB99

CPT Code(s):


Collection Procedure:

Serum gel tubes should be centrifuged within 2 hours of collection.

Specimen Requirements:

Type: Peripheral blood


  • Serum, gel
  • Plasma, gel

Sample Volume: 0.5 mL serum

Stability (collection to time of analysis/testing):

  • Refrigerated: 2 weeks
  • Frozen: 1 year

Unacceptable Conditions: Moderate-to-gross hemolysis

Specimen Retention Time: 5 days


The lipase procedure is based on the colorimetric method. Pancreatic lipase hydrolyzes esters of long chain fatty acids from their triglycerides. The enzyme activity requires the presence of co-lipase. 1,2-Diglyceride is hydrolyzed to 2-monoglyceride and fatty acid. The 2-monoglyceride is then measured by coupled enzyme reactions catalyzed by monoglyceride lipase (MGLP), glycerol kinase (GK), glycerol phosphate oxidase (GPO) and peroxidase (POD).


Within 24 hours

Reference Values:

11 – 82 U/L