Additional Information:
UFHPL Epic order code: LAB99
CPT Code(s):
83690
Collection Procedure:
Serum gel tubes should be centrifuged within 2 hours of collection.
Specimen Requirements:
Type: Peripheral blood
Container/Tube:
- Serum, gel
- Plasma, gel
Sample Volume: 0.5 mL serum
Stability (collection to time of analysis/testing):
- Refrigerated: 2 weeks
- Frozen: 1 year
Unacceptable Conditions: Moderate-to-gross hemolysis
Specimen Retention Time: 5 days
Methodology:
The lipase procedure is based on the colorimetric method. Pancreatic lipase hydrolyzes esters of long chain fatty acids from their triglycerides. The enzyme activity requires the presence of co-lipase. 1,2-Diglyceride is hydrolyzed to 2-monoglyceride and fatty acid. The 2-monoglyceride is then measured by coupled enzyme reactions catalyzed by monoglyceride lipase (MGLP), glycerol kinase (GK), glycerol phosphate oxidase (GPO) and peroxidase (POD).
Reported:
Within 24 hours
Reference Values:
Adults: 11 – 82 U/L