Micro Array: Comparative Genomic Hybridization (aCGH) with SNP

CPT Code(s):

81229

Specimen Requirements:

*Please note:  If ordering both a chromosome analysis/FISH study AND a microarray analysis, please draw two separate tubes of blood. Chromosomes/FISH studies require as close to 2.0ml+ as possible, collected in a dark green sodium heparin tube. Microarrays require the same volume (~2.0ml+) and the preferred tube is a lavender EDTA, but a dark green sodium heparin is also acceptable. Further details for UF PathLab’s cytogenetics requirements are available here and LabCorp microarray requirements are available here.

Peripheral Blood: Peripheral blood must be collected aseptically in sodium heparin tubes (usually green-top tubes) and immediately rotated thoroughly to prevent clotting. For infants, obtain approximately 2 cc. Obtain 5 – 7 cc for children and adults.

Note: A separate blood sample is required if a concurrent conventional blood chromosome and/or FISH studies are desired.

Do not freeze or fix samples in any manner contrary to the maintenance of cell viability. Maintain sample sterility; keep all specimens at room temperature; and deliver samples to the laboratory within 24 – 48 hours. Ship specimens at room temperature for overnight delivery.

  • Unacceptable Conditions:
    • Frozen specimens
    • Specimens greater than 48 hours old
  • Stability (collection to initiation of testing):
    • Ambient: 24 – 48 hours
    • Refrigerated: Unacceptable

Use:

This test provides a higher resolution level compared to chromosome-based (CGH) testing for genomic copy number variations.

Methodology:

Oligonucleotide (or oligonucleotide with SNP) genomic microarray platform; requires cell culture

Reported:

14 – 28 days

Reference Values:

By report; interpretive