T-Cell Clonality (T-Cell Receptor Gamma (TCRG)) Gene Rearrangements Analysis

Additional Information:

T Cell Clonality Analysis

Epic Order Code: LAB5639

CPT Code(s):

  • 81342
  • 88381 (FFPE)

Specimen Requirements:

Acceptable sample type: The samples should be specifically chosen by a pathologist to include at least 5 % tumor cells without significant necrosis or inflammation. The following types of samples are accepted:

a. FFPE (formalin fixed, paraffin embedded) block
b. Unstained slides (USS) from FFPE
c. Bone marrow aspirate
d. Peripheral blood

Formalin Fixed Parafin Embedded (FFPE) specimens or Unstained Slides (USS) prepared from FFPE specimens must be accompanied by 1 corresponding H&E from the same block with the tumor circled by a qualified pathologist AND the estimated percentage of tumor cells in the circled area(s). For Peripheral Blood (PB) or Bone Marrow Aspirate (BMA), only the estimated percent of tumor cells is required.
Bone marrow aspirate or Peripheral blood:
Container/Tube: Lavender top (EDTA)
Specimen Volume: 3mL
Collection Instructions:
1. Invert several times to mix blood.
2. Send whole blood specimen in original tube. Do not aliquot. Do not freeze or centrifuge.
Specimen Stability Information: Ambient 24 Hours/ Refrigerated 1 Week/ Frozen Unacceptable
Transport to the laboratory as soon as possible for testing.
Unacceptable conditions:
Clotted or frozen blood.
Specimens received beyond recommended stability.
Less than the minimum volume requirements for blood collection.
Failure to indicate tumor and/or percent tumor will result in delayed specimen processing or specimen rejection.
Tumor percent cannot be zero (0) or unknown.
A percent tumor of less than 5 will be reviewed on a case by case basis by the director of molecular pathology.

Use:

T-cell receptor (TCRG) Gene Rearrangement Analysis is a qualitative assay used to detect clonal rearrangements in a T-cell population from genomic DNA.

Methodology:

PCR and Fragment Analysis

Reported:

5 – 10 business days

Reference Values:

Normal: Polyclonal
Abnormal: Monoclonal/Clonal/Oligoclonal

Test Classification:

Laboratory Developed Test