Testosterone Total and Free (with SHBG)

Additional Information:

UFHPL Epic order code: LAB839

CPT Code(s):

84402, 84403, 84270

Collection Procedure:

Serum must be separated from cells within 45 minutes of venipuncture. Send serum in a plastic transport tube.

Specimen Requirements:

Important: Indicate the patient’s age and gender when ordering this test.

Type: Serum only, FROZEN

Container/Tube: Red-top tube or gel-barrier tube

  • If a red-top tube is used, transfer the separated serum to a plastic transport tube.

Sample Volume: 2 mL

Storage: FREEZE

  • Stability: Room temperature up to 2 days
  • Refrigerated up to 2 days
  • Frozen up to 600 days


This test is used to evaluate hirsutism and masculinization in women and testicular function in clinical states where the testosterone-binding proteins may be altered (obesity, cirrhosis, thyroid disorders).


A pediatric reference interval has not been established for the direct free testosterone test.


High-pressure liquid chromatography (HPLC) tandem mass spectrometry; equilibrium dialysis; electrochemiluminescence immunoassay (ECLIA)


6-12 days