Additional Information:
UFHPL Epic order code: LAB134
CPT Code(s):
Specimen Requirements:
Type: Peripheral blood
- Serum, gel
- Plasma, gel
Sample Volume: 0.5 mL
Preparation: Fasting for ≥12 hours
- Refrigerated: 7 days
- Frozen: 3 months
Unacceptable Conditions: Moderate-to-gross hemolysis
Methodology:
This triglyceride procedure is based on a series of coupled enzymatic reactions. The triglycerides in the sample are hydrolyzed by a combination of microbial lipases to give glycerol and fatty acids. The glycerol is phosphorylated by adenosine triphosphate (ATP) in the presence of glycerol kinase (GK) to produce glycerol-3-phosphate. The glycerol-3-phosphate is oxidized by molecular oxygen in the presence of GPO (glycerol phosphate oxidase) to produce hydrogen peroxide (H2O2) and dihydroxyacetone phosphate. The formed H2O2 reacts with 4-aminophenazone and N,N-bis(4-sulfobutyl)-3,5-dimethylaniline, disodium salt (MADB) in the presence of peroxidase (POD) to produce a chromophore, which is read at 660/800nm. The increase in absorbance at 660/800 nm is proportional to the triglyceride content of the sample.
Reported:
Within 24 hours
Reference Values:
|
Triglyceride |
Risk Classification |
|
< 150 mg/dL |
Normal |
|
150 – 199 mg/dL |
Borderline high |
|
200 – 499 mg/dL |
High |
|
≥ 500 mg/dL |
Very high |