Additional Information:

UFHPL Epic order code: LAB134

CPT Code(s):


Specimen Requirements:

Type: Peripheral blood


  • Serum, gel
  • Plasma, gel

Sample Volume: 0.5 mL

Preparation: Fasting for ≥12 hours

Stability (collection to time of analysis/testing):

  • Refrigerated: 7 days
  • Frozen: 3 months

Unacceptable Conditions: Moderate-to-gross hemolysis

Specimen Retention Time: 5 days


This triglyceride procedure is based on a series of coupled enzymatic reactions. The triglycerides in the sample are hydrolyzed by a combination of microbial lipases to give glycerol and fatty acids. The glycerol is phosphorylated by adenosine triphosphate (ATP) in the presence of glycerol kinase (GK) to produce glycerol-3-phosphate. The glycerol-3-phosphate is oxidized by molecular oxygen in the presence of GPO (glycerol phosphate oxidase) to produce hydrogen peroxide (H2O2) and dihydroxyacetone phosphate. The formed H2O2 reacts with 4-aminophenazone and N,N-bis(4-sulfobutyl)-3,5-dimethylaniline, disodium salt (MADB) in the presence of peroxidase (POD) to produce a chromophore, which is read at 660/800nm. The increase in absorbance at 660/800 nm is proportional to the triglyceride content of the sample.


Within 24 hours

Reference Values:


Risk Classification

< 150 mg/dL


150 – 199 mg/dL

Borderline high

200 – 499 mg/dL


≥ 500 mg/dL

Very high