Vitamin D, 25-Hydroxy

Additional Information:

Epic order code: LAB21059501

  • This test is not the same as Calcitriol (1,25 di-OH Vitamin D) [081091] (vitamin D3), which must be ordered separately.
  • CPT Code(s):


    Specimen Requirements:

    Collect at least 1 mL blood in a red-top (preferred) or gel barrier serology tube. Centrifuge the blood and remove the serum/plasma from the cells within two hours of collection.

    Transport/Storage Temperature:

    • Ambient or Refrigerated
    Stability (collection to shipping of the samples):

    • Ambient: 7 days
    • Refrigerated: 14 days
    • Frozen (-20°C): 14 days


    This test aids in:

    • The diagnosis of vitamin D deficiency and/or hypervitaminosis D;
    • Potential differential diagnosis of rickets and osteomalacia; and
    • Tracking vitamin D replaement therapy.


    Immunochemiluminometric assay (ICMA).


    1 – 3 days

    Reference Values:

    30−100 ng/mL