Amino Acids

CPT Code(s):



Liquid chromatography - tandem mass spectrometry (LC-MS/MS) after derivation by benzoyl chloride


Monday - Friday; STAT is available after consultation with the Metabolics Laboratory director; call 352.265.9900 to make arrangements for a STAT case.


Within 5 business days


This test is used to evaluate and monitor amino acidemias, as well as monitor patients on amino acid- and protein-restricted diets.

Reports include data on amino acids used for the diagnosis and monitoring of inherited metabolic disorders.

Specimen Requirements:

Type: Blood
Container: Heparin is preferred, but EDTA is acceptable.
Sample volume: 0.5 mL
Minimum volume: 0.25 mL
Storage: Refrigerate whole blood specimens.
Stability (collection to time of analysis):

  • Ambient: 2 hours
  • Refrigerated: 1 day
  • Frozen: 4 weeks
Rejection criteria:

  • Frozen whole blood
  • Citrate or acid citrate dextrose (ACD) tubes

Collection Procedure:

Special instructions:

  1. Venipuncture is preferred. Capillary specimens are acceptable, but results for several amino acids *serine, glycine, glutamine, glutamic acid) may not reflect plasma amino acid concentrations.
  2. Spin specimens before refrigerating them, if blood is collected in a gel tube (PST, PPT).
  3. If transport is delayed or specimens are sent by overnight courier, spin blood and seperate and freeze plasma.

Reference Values:


Interpretation Data:



UFHPL Test #: 40015

UFHPL Epic order code: LAB811

Reported Notes: