Creatinine – CREA

CPT Code(s):

82565

Methodology:

This Creatinine procedure is a kinetic modification of the Jaffe procedure, in which creatinine reacts with picric acid at alkaline pH to form a yellow orange complex. However, this reaction is not completely specific for creatinine since other reducing substances such as glucose, pyruvate, ascorbic acid, and acetoacetates will react with picrate to form a similar color. Fabiny and Ertingshausen found that alkaline creatinine picrate reaches maximum color development at a different rate than pseudo-creatinine material. Cook utilized different reaction rates of alkaline picrate positive substances to obtain greater specificity with the Jaffe reaction. The rate of change in absorbance at 520/800nm is proportional to the creatinine concentration in the sample.

Performed:

Monday - Friday

Reported:

Within 24 hours

Use:

-

Specimen Requirements:

Type: Peripheral blood

Container/Tube:

  • Serum, gel
  • Plasma, gel

Sample Volume: 0.5mL serum

Stability (collection to time of analysis/testing):

  • Refrigerated: 7 days
  • Frozen: 1 year

Unacceptable Conditions: Moderate and gross hemolysis

Specimen Retention Time: 5 days

Collection Procedure:

-

Reference Values:

-

Interpretation Data:

-

Notes:

UFHPL Test #: 20140

UFHPL Epic order code: LAB66

Reported Notes:

-

Result:

-