FISH Study: Neoplastic Interphase MLL

CPT Code(s):

  • 88271
  • 88275
  • 88291

Methodology:

Interphase nuclei fluorescence in-situ hybridization (FISH) break-apart rearrangement analyses; a concurrent or previous conventional cytogenetic workup is required/recommended (where possible)

Performed:

Monday - Saturday

Reported:

5 - 12 days

Use:

This test is used to detect rearrangements involving the MLL gene locus at 11q23, which may result in the formation of a large variety of hybrid gene fusion products and are seen in ALL, AML and bi-phenotypic acute leukemias.

Specimen Requirements:

  • Bone Marrow Aspirate: Obtain 1 - 2 cc of in a green-top sodium heparin tube and immediately rotate it thoroughly to ensure adequate mixing with the anticoagulant.
  • Bone Marrow Core Biopsy: Deposit samples in a sterile container containing tissue culture medium with antibiotics (RPMI) that have been brought to room temperature before collection.*
  • Peripheral Blood Specimens: Peripheral blood specimens may be submitted in those clinical conditions (i.e. circulating blasts) and/or circumstances (where warranted).

Do not freeze or fix samples in any manner contrary to the maintenance of cell viability. Maintain sample sterility; keep all specimens at room temperature; and deliver samples to the laboratory within 24 - 48 hours. Ship specimens at room temperature for overnight delivery.

Collection Procedure:

-

Reference Values:

By report; interpretive

Interpretation Data:

-

Notes:

UFHPL Test #: 30225

Reported Notes:

-

Result:

Interpretive report