GatorSeq (NGS Panel)

GatorSeq is a hybridization capture-based next-generation-targeted resequencing assay for deep sequencing of 177 key cancer genes. Tumor samples are macrodissectedto increase tumor cell percentage in the analyzed sample and sequenced on an Illumina NextSeq to high-uniform depth (targeting 500x coverage by non-polymerase chain reaction- (PCR) duplicate read pairs, with more than 99 percent of exons at coverage greater than 100 times). Sequence data are processed using a customized analysis pipeline that is designed to accurately detect base substitutions and insertions/deletions. Annotated reports are generated using a GenomeOncology-developed software and database.

• Less than 10 percent tumor
• Specimens fixed/processed in alternative fixatives (alcohol, Anatech Prefer fixative) or heavy metal fixatives
• Decalcified specimens

• Frozen (on Dry Ice): 24 - 48 hours