GatorSeq (NGS)
CPT Code(s): |
81455
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Methodology: |
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Performed: |
Monday - Friday |
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Reported: |
10 - 14 days |
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Use: |
This test is used to determine a prognosis and/or treatment options for individuals with cancer at initial diagnosis or with refractory disease.
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Specimen Requirements: |
The preferred specimen is formalin-fixed, paraffin-embedded tissue block; however, fresh tissue or cell suspensions are also acceptable.
Shipping for Fresh/Fresh-Frozen Tissue: Flash-freeze and ship frozen specimens on dry ice for overnight arrival Monday through Friday. Unacceptable Conditions:
Stability (collection to initiation of testing):
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Collection Procedure: |
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Reference Values: |
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Interpretation Data: |
No mutation detected; interpretative report provided
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Notes: |
UFHPL Test #: 10145 GatorSeq is a hybridization capture-based next-generation-targeted resequencing assay for deep sequencing of 115 key cancer genes. Tumor samples are macrodissectedto increase tumor cell percentage in the analyzed sample and sequenced on an Illumina NextSeq to high-uniform depth (targeting 500x coverage by non-polymerase chain reaction- (PCR) duplicate read pairs, with more than 99 percent of exons at coverage greater than 100 times). Sequence data are processed using a customized analysis pipeline that is designed to accurately detect base substitutions and insertions/deletions. Annotated reports are generated using a GenomeOncology-developed software and database. The entire coding sequences of the following genes are covered by this assay:
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Reported Notes: |
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Result: |
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