GatorSeq (NGS)

81455

This test is used to determine a prognosis and/or treatment options for individuals with cancer at initial diagnosis or with refractory disease.

The preferred specimen is formalin-fixed, paraffin-embedded tissue block; however, fresh tissue or cell suspensions are also acceptable.

Unacceptable Conditions:

• Less than 10 percent tumor
• Specimens fixed/processed in alternative fixatives (alcohol, Anatech Prefer fixative) or heavy metal fixatives
• Decalcified specimens

Stability (collection to initiation of testing):

• Frozen (on Dry Ice): 24 - 48 hours

No mutation detected; interpretative report provided

GatorSeq is a hybridization capture-based next-generation-targeted resequencing assay for deep sequencing of 115 key cancer genes. Tumor samples are macrodissectedto increase tumor cell percentage in the analyzed sample and sequenced on an Illumina NextSeq to high-uniform depth (targeting 500x coverage by non-polymerase chain reaction- (PCR) duplicate read pairs, with more than 99 percent of exons at coverage greater than 100 times). Sequence data are processed using a customized analysis pipeline that is designed to accurately detect base substitutions and insertions/deletions. Annotated reports are generated using a GenomeOncology-developed software and database.