Alanine Amino Transferase (ALT)

Additional Information:

UFHPL Epic order code: LAB132

CPT Code(s):

84460

Collection Procedure:

Serum gel tubes should be centrifuged within 2 hours of collection.

Specimen Requirements:

Type: Peripheral blood

Container/Tube:

  • Serum, gel
  • Plasma, gel
Sample Volume: 0.5 mL

Stability (collection to shipping of the sample):

  • Refrigerated: 3 days
  • Frozen: > 3 days

Unacceptable Conditions: Moderate-to-gross hemolysis

Specimen Retention Time: 5 days

Methodology:

This ALT procedure is based on principles outlined by Wroblewski and LaDue and utilizes a modification of the methodology recommended by the International Federation of Clinical Chemistry (IFCC). ALT transfers the amino group from alanine to a-oxoglutarate to form pyruvate and glutamate. The pyruvate enters a lactate dehydrogenase (LD) catalyzed reaction with NADH to produce lactate and NAD+. The decrease in absorbance due to the consumption of NADH is measured at 340nm and is proportional to the ALT activity in the sample

Reported:

Within 24 hours

Reference Values:

Female: 0-45 IU/L

Male: 0-50 IU/L

Performed:

Monday - Friday