Additional Information:
UFHPL Epic order code: LAB132
CPT Code(s):
Collection Procedure:
Specimen Requirements:
- Serum, gel
- Plasma, gel
- Refrigerated: 3 days
- Frozen: > 3 days
Unacceptable Conditions: Moderate-to-gross hemolysis
Methodology:
This ALT procedure is based on principles outlined by Wroblewski and LaDue and utilizes a modification of the methodology recommended by the International Federation of Clinical Chemistry (IFCC). ALT transfers the amino group from alanine to a-oxoglutarate to form pyruvate and glutamate. The pyruvate enters a lactate dehydrogenase (LD) catalyzed reaction with NADH to produce lactate and NAD+. The decrease in absorbance due to the consumption of NADH is measured at 340nm and is proportional to the ALT activity in the sample
Reported:
Within 24 hours
Reference Values:
Male: 0-50 IU/L