Additional Information:
UFHPL Epic order code: LAB210768
References
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CPT Code(s):
86225, 86235 (x 8)
Specimen Requirements:
Type: Serum
Container/Tube: Red-top tube or gel-barrier tube
Sample Volume: 1 mL
Minimum Volume: 0.5 mL (Repeat testing is not possible with this specimen volume.)
Storage: Store specimens at room temperature.
Stability (collection to time of analysis/testing):
- Ambient: 14 days
- Refrigerated: 14 days
- Frozen: 14 days
- Freeze/Thaw Cycles: Stable (x 3)
Rejection Criteria:
- Hemolysis
- Lipemia
- Gross bacterial contamination
- Icterus
Use:
This test is used to detect antibodies associated with SLE and mixed connective tissue disease. Anti-Sm is highly specific for SLE. Anti-RNP is found with a variety of rheumatoid diseases. RNP, Sm, SS-A, SS-B, Scl-70, and Jo-1 are extractable nucleoprotein complexes found in the nucleus and/or cytoplasm of substrate cells.
The test includes:
- Anticentromere B antibodies
- Anti-dsDNA
- Antichromatin antibodies
- Anti-Jo-1; RNP antibodies
- Antiscleroderma 70 antibodies
- Smith antibodies
- Sjögren anti-SS-A
- Sjögren anti-SS-B
Methodology:
Multiplex flow immunoassay