Additional Information:
UFHPL Epic order code: LAB2058
CPT Code(s):
87116
87206
87015
Collection Procedure:
Three (3) respiratory specimens should be collected at 8- to 24-hour intervals (24-hour interval is preferred) and should include at least 1 first-morning specimen.
Neutralize Gastric washing specimens within 4 hours of collection with 100 mg of sodium carbonate per 5 to 10 mL of specimen.
Specimen Requirements:
Sputum
Container: sterile leak-proof sputum collection device.
Specimen volume: minimum 5 mL
***
Bronchial alveolar lavage (BAL)
Container: sterile leak-proof container. Specimen volume: minimum 5 ml.
***
Gastric lavage obtained from children: Container: sterile leak-proof container. Specimen volume: minimum 10 ml.
***
Body Fluid
Container: sterile leak-proof container. Specimen volume: minimum 1.5 ml.
***
Stool
Container: sterile leak-proof container. Specimen volume: minimum 5-10 g.
***
Tissue (not in formalin)
Container: sterile leak-proof container. Specimen volume: minimum 5-10 mm.
***
Urine
Container: sterile leak-proof container. Specimen volume: minimum 5 ml.
***
Specimens should be transported as quickly as possible to the laboratory, preferably within one hour. Specimens delayed longer than 30 minutes before transport should be refrigerated.
Use:
Detection and identification of Mycobacterium species.
Methodology:
Continuous monitoring broth culture system and conventional culture.
Includes microscopic exam with fluorochrome stain.
Reported:
42 days (Positive cultures reported when detected)
Reference Values:
No growth.
Identification (plus susceptibility if indicated) performed on positive cultures.
Interpretation Data:
A final negative report is issued after 42 days of incubation.
Positive cultures are reported when detected.