Culture AFB with Stain

Additional Information:

UFHPL Epic order code: LAB2058

CPT Code(s):

87116
87206
87105

Collection Procedure:

Three (3) respiratory specimens should be collected at 8- to 24-hour intervals (24-hour interval is preferred) and should include at least 1 first-morning specimen.
Neutralize Gastric washing specimens within 4 hours of collection with 100 mg of sodium carbonate per 5 to 10 mL of specimen.

Specimen Requirements:

Sputum
Container: sterile leak-proof sputum collection device.
Specimen volume: minimum 5 mL
***
Bronchial alveolar lavage (BAL)
Container: sterile leak-proof container. Specimen volume: minimum 5 ml.
***
Gastric lavage obtained from children: Container: sterile leak-proof container. Specimen volume: minimum 10 ml.
***
Body Fluid
Container: sterile leak-proof container. Specimen volume: minimum 1.5 ml.
***
Stool
Container: sterile leak-proof container. Specimen volume: minimum 5-10 g.
***
Tissue (not in formalin)
Container: sterile leak-proof container. Specimen volume: minimum 5-10 mm.
***
Urine
Container: sterile leak-proof container. Specimen volume: minimum 5 ml.
***
Specimens should be transported as quickly as possible to the laboratory, preferably within one hour. Specimens delayed longer than 30 minutes before transport should be refrigerated.

Use:

Detection and identification of Mycobacterium species.

Methodology:

Continuous monitoring broth culture system and conventional culture.
Includes microscopic exam with fluorochrome stain.

Reported:

42 days (Positive cultures reported when detected)

Reference Values:

No growth.

Identification (plus susceptibility if indicated) performed on positive cultures.

Performed:

TAT 42 days
For inquiries, email NTMlab@pathology.ufl.edu

Interpretation Data:

A final negative report is issued after 42 days of incubation.
Positive cultures are reported when detected.

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