FLT3 Gene Mutation Analysis
Collect blood or bone marrow in a lavender-top (EDTA) tube. Refrigerate and send intact specimen(s) within 48 hours of collection.
- Paraffin block specimens
The FLT3 gene encodes a cell surface receptor tyrosine kinase that is expressed on early hematopoietic stem cells. Activating mutations in FLT3 occur in approximately 25 to 30 percent of patients with acute myelogenous leukemia (AML). The mutation generally occurs as either an internal tandem duplication (ITD) within the juxtamembrane domain, or as a missense point mutation within the tyrosine kinase domain (TKD) at codon 835. This test detects both the FLT3-ITD and FLT3-TKD (codon 835) mutations. The presence of a FLT3-ITD mutation has been associated with a significantly poorer outcome in normal karyotype AML.
Multiplex polymerase chain reaction (PCR) with capillary electrophoresis
Within 7 days
Monday - Friday