CPT Code(s):
81245
Additional Information:
Epic Order Code: LAB56210
Note: FLT3 mutations other than ITD will not be detected with this assay. For FLT3-TKD, please also order GatorseqNGS Panel (LAB5622).
Specimen Requirements:
Acceptable sample type: The samples should be specifically chosen by a pathologist to include at least 5 % tumor cells. Please provide percent tumor when ordering. The following are the types of samples we can accept:
a. Bone marrow aspirate
b. Peripheral blood
Container/Tube: Lavender top (EDTA)
Specimen Volume: 3mL
Collection Instructions:
1. Invert several times to mix blood.
2. Send whole blood specimen in original tube. Do not aliquot. Do not freeze or centrifuge.
Specimen Stability Information: Ambient 24 Hours/ Refrigerated 1 Week/ Frozen Unacceptable
Transport to the laboratory as soon as possible for testing.
Unacceptable conditions:
Clotted or frozen blood.
Specimens received beyond recommended stability.
Less than the minimum volume requirements for blood collection.
FFPE or USS from FFPE are not acceptable sample types for FLT3-ITD analysis.
A percent tumor of less than 5 will be reviewed on a case by case basis by the director of molecular pathology
Use:
This is a qualitative assay designed to detect mutations of the FLT3 receptor by internal tandem duplication (ITD) in acute myeloid leukemia (AML). The principal use for the FLT3 internal tandem duplication assay is to detect the presence of mutations in this gene in a patient sample in which acute myelogenous leukemia (AML) has been diagnosed. It is not intended to be used to detect minimal residual disease.This assay should be ordered in conjunction with the GatorSeq NGS Panel to account for the FLT3 TKD portion.
Methodology:
PCR and Fragment Analysis
Reported:
5-10 business days
Reference Values:
FLT3 ITD not detected.
Test Classification:
Laboratory Developed Test