GatorSeq NGS Panel

Additional Information:

The entire coding sequences of the following genes are covered by this assay:

Genes tested for copy number alterations: ALK, ATM, ATRX, BAP1, BRAF, BRCA1, BRCA2, CCND1, CCND3, CCNE1, CDK4, CDK6, CDKN2A, CDKN2B, CHEK1, CIC, CTNNB1, EGFR, ERBB2, ERBB3, ESR1, FGF10, FGF14, FGF19, FGF23, FGF3, FGF4, FGF6, FGFR1, FGFR2, FGFR3, FGFR4, FUBP1, HRAS, IDH2, JAK2, KIT, KRAS, MDM2, MDM4, MET, MTAP, MYC, MYCN, NF1, NF2, NRAS, PDGFRA, PDGFRB, PIK3CA, PIK3CB, PTCH1, PTEN, RAF1, RB1, RET, RICTOR, SETD2, SMO, TERT, TFEB, TP53

Specimen Requirements:

Acceptable sample type: The samples should be specifically chosen by a pathologist to include at least 5 % tumor cells without significant necrosis or inflammation. The following types of samples are accepted:

a. FFPE (formalin fixed, paraffin embedded) block
b. Unstained slides (USS) from FFPE
c. Bone marrow aspirate
d. Peripheral blood
Formalin Fixed Parafin Embedded (FFPE) specimens
Must be accompanied by 1 corresponding H&E from the same block with the tumor circled by a qualified pathologist AND the estimated percentage of tumor cells present in the circled area(s).
1. Circle the area(s) on the H&E slide where tumor cells are present with a marking pen.
2. Write the percent tumor on the H&E slide with a marking pen.

Unstained Slides (USS) prepared from FFPE specimens
For 10mmx10mm sized tissue or larger, send 10 USS. For smaller tissues, send 20 USS
Must be accompanied by 1 corresponding H&E from the same block with the tumor circled by a qualified pathologist AND the estimated percentage of tumor cells present in the circled area(s).
1. Circle the area(s) on the H&E slide where tumor cells are present with a marking pen.
2. Write the percent tumor on the H&E slide with a marking pen.

Bone marrow aspirate or Peripheral blood
Container/Tube: Lavender top (EDTA)
Specimen Volume: 3mL
Collection Instructions:
1. Invert several times to mix blood.
2. Send whole blood specimen in original tube. Do not aliquot. Do not freeze or centrifuge.
Specimen Stability Information: Ambient 24 Hours/ Refrigerated 1 Week/ Frozen Unacceptable
Transport to the laboratory as soon as possible for testing.

Unacceptable conditions:
Tissues that are not fixed in 10% formalin, or has been fixed for longer than 3 days in 10% formalin.
Clotted or frozen blood.
Specimens received beyond recommended stability.
Less than the minimum volume requirements for blood collection.
Failure to indicate tumor and/or percent tumor will result in delayed specimen processing or specimen rejection.
Tumor percent cannot be zero (0) or unknown.
A percent tumor of less than 5 will be reviewed on a case by case basis by the director of molecular pathology.”

Use:

Reported:

10 – 14 days

Reference Range: