Monkeypox (Orthopoxvirus), DNA, PCR

Additional Information:

UFHPL Epic order code: LAB170059

CPT Code(s):


Collection Procedure:

Vigorously swab or brush the base of the lesion with a sterile dry polyester, rayon or Dacron swab. Insert the swab into the tube containing UTM or VTM. Carefully break the swab at the scoreline and tightly close the sample. Some UTM kits may contain two swabs; however, only one swab needs to be collected and submitted for testing. If multiple lesions with differing appearances are present, consider submitting an additional UTM/VTM collection, as described above, for each lesion.

Specimen Requirements:

Lesion swab

One swab in UTM or VTM

Minimum Volume
One swab in UTM or VTM

Universal Transport Media (UTM) or Viral Transport Media (VTM)

Stability Requirements

  • Refrigerated: 7 days (stability provided by manufacturer or literature reference)
  • Frozen: 30 days (stability provided by manufacturer or literature reference)


Vesicular specimens collected from persons infected with a non-variola Orthopoxvirus (such as vaccinia, monkeypox, or cowpox) are expected to produce a positive result with this assay. Although this assay does not differentiate vaccinia or monkeypox virus from cowpox, camelpox, ectomelia or gerbilpox virus, a positive result with this assay in the United States is most likely due to monkeypox virus or vaccinia virus; however, potential exposure to other Orthopoxviruses should be considered.


Polymerase chain reaction (PCR)


3-4 days