Paroxysmal Nocturnal Hemoglobinuria (PNH) – CD55/59 Erythrocytes, FLAER Granulocytes/Monocytes » Pathology Laboratories » College of Medicine

CPT Code(s):

88184
88185
88186
88187
88188
88189

Specimen Requirements:

2 x 5 – 10 mL peripheral blood in a lavender-top tube (EDTA)
Peripheral blood only; it must be received in the lab within 24 of collection hours, but no later than 72 hours.

Specimen Storage/Transport Temperature: The fresh peripheral blood specimen can be transported on cold pack or wet ice, but do not fix or freeze specimens (no dry ice).

Unacceptable Conditions

Specimens greater than 24 hours old

Stability (collection to initiation of testing):

Ambient: 24 hours
Refrigerated: 24 hours
Frozen: Unacceptable

For more information, contact the UF Health Pathology Laboratories Client Services Department at 888.375.LABS (5227).

Use:

This test is used to diagnose PNH and quantify PNH clones in blood. PNH may be associated with hemolytic anemia, leukopenia, thrombocytopenia and aplastic anemia. This method uses expression of CD55/59 on erythrocytes and the more sensitive fluorescent aerolysin (FLAER) on granulocytes/monocytes.

Methodology:

Flow cytometry

Reported:

1 – 3 days

Reference Values:

Negative; presence of PNH clone is reported and quantified using CD55/59(for RBCS), FLAER for granulocytes and monocytes