Tissue Transglutaminase (tTG), IgG

Additional Information:

UFHPL Epic order code: LAB721


  • Dieterich W, Ehnis T, Bauer M, et al. Identification of tissue transglutaminase as the autoantigen of celiac disease. Nat Med. 1997 Jul; 3(7):797-801. PubMed 9212111
  • Volta U, Molinaro N, De Franchis R, et al. Correlation between IgA antiendomysial antibodies and subtotal villous atrophy in dermatitis herpetiformis. J Clin Gastroenterol. 1992 Jun; 14(4):298-301. PubMed 1607605

CPT Code(s):


Specimen Requirements:

Important: Specimens should be free of bacterial contamination, hemolysis and lipemia.

Type: Serum

Container/Tube: Red-top tube or gel-barrier tube

Sample Volume: 1 mL

Minimum Volume: 0.3 mL (Repeat testing is not possible with this specimen volume.)

Storage: Refrigerate specimens after collection.

Stability (collection to time of analysis/testing):

  • Ambient: 14 days
  • Refrigerated: 14 days
  • Frozen: 14 days
  • Freeze/Thaw cycles: Stable (x3)
Rejection Criteria:

  • Gross bacterial contamination
  • Lipemia
  • Hemolysis


This test is used to detect antibodies to aid in the diagnosis of gluten-sensitive enteropathy (GSE), such as celiac disease and dermatitis herpetiformis.


A negative result does not rule out celiac disease. The inclusion of the tTG IgG autoantibodies is important because it is not uncommon for celiac patients to be IgA deficient. Results of this assay should be used in conjunction with clinical findings and other serological tests.


Enzyme immunoassay (EIA)

Reference Values:

Reference Intervals

  • Negative: < 6 units/mL
  • Weak-positive: 6 – 9 units/mL
  • Positive: > 9 units/mL